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Apical-out enteroids for pathogen access
Suspending human enteroids without ECM flips polarity so microbes can meet the apical face.
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What model is developed?
Human enteroids with controllable polarity.
Source: Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions · DOI · quote verified
What cue orients polarity?
ECM / β1 integrin signaling.
Source: Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions · DOI · quote verified
What culture change flips polarity?
Suspension without basement-membrane extract.
Source: Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions · DOI · quote verified
Why does apical-out matter?
Apical surface becomes experimentally accessible.
Source: Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions · DOI · quote verified
What tissue does this model?
Human intestinal epithelium.
Source: Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions · DOI · quote verified
What biological process is studied?
Epithelial polarity orientation.
Source: Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions · DOI · quote verified
What geometry are enteroids?
3D spheroid cultures.
Source: Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions · DOI · quote verified
Are stem cells involved?
Yes — Lgr5+ intestinal stem-cell cultures.
Source: Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions · DOI · quote verified
What infection use-case?
Apical host–pathogen challenges.
Source: Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions · DOI · quote verified
Student takeaway?
ECM cues set gut polarity for pathogen studies.
Source: Controlling Epithelial Polarity: A Human Enteroid Model for Host-Pathogen Interactions · DOI · quote verified