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SNARE timing in sperm acrosome exocytosis
Sperm acrosome exocytosis proceeds via ordered SNARE assembly/disassembly around intra-acrosomal Ca2+ release.
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Cellular event?
Sperm acrosomal exocytosis.
Source: Dynamics of SNARE assembly and disassembly during sperm acrosomal exocytosis · DOI · quote verified
Core fusion machinery?
SNARE proteins and regulators.
Source: Dynamics of SNARE assembly and disassembly during sperm acrosomal exocytosis · DOI · quote verified
GTPase before Ca2+?
Rab3A required upstream.
Source: Dynamics of SNARE assembly and disassembly during sperm acrosomal exocytosis · DOI · quote verified
What releases later?
Intra-acrosomal Ca2+ efflux.
Source: Dynamics of SNARE assembly and disassembly during sperm acrosomal exocytosis · DOI · quote verified
After Ca2+ sensors?
Synaptotagmin and SNAREs finish fusion.
Source: Dynamics of SNARE assembly and disassembly during sperm acrosomal exocytosis · DOI · quote verified
Disassembly factors?
NSF/α-SNAP remodel SNARE complexes.
Source: Dynamics of SNARE assembly and disassembly during sperm acrosomal exocytosis · DOI · quote verified
Tool to stage Ca2+?
Photolabile NP-EGTA-AM chelator.
Source: Dynamics of SNARE assembly and disassembly during sperm acrosomal exocytosis · DOI · quote verified
Antibody evidence?
Anti-Rab3A blocks only if added before Ca2+ challenge.
Source: Dynamics of SNARE assembly and disassembly during sperm acrosomal exocytosis · DOI · quote verified
cis then trans?
cis SNAREs disassemble then loose trans complexes form.
Source: Dynamics of SNARE assembly and disassembly during sperm acrosomal exocytosis · DOI · quote verified
Takeaway?
Exocytosis is a timed Rab→SNARE→Ca2+ sequence.
Source: Dynamics of SNARE assembly and disassembly during sperm acrosomal exocytosis · DOI · quote verified