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CRISPR repeats conserve RNA-like structure
CRISPR repeats across microbes form clusters with conserved sequence and predicted RNA secondary structure.
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What elements were analyzed?
CRISPR repeats and spacers.
Source: Evolutionary conservation of sequence and secondary structures in CRISPR repeats · DOI · quote verified
How many genomes?
439 bacterial/archaeal genomes.
Source: Evolutionary conservation of sequence and secondary structures in CRISPR repeats · DOI · quote verified
Arrays found?
561 arrays in 195 genomes.
Source: Evolutionary conservation of sequence and secondary structures in CRISPR repeats · DOI · quote verified
Genome prevalence?
About 44% of tested genomes.
Source: Evolutionary conservation of sequence and secondary structures in CRISPR repeats · DOI · quote verified
Why structure?
Palindromic repeats suggest RNA folds.
Source: Evolutionary conservation of sequence and secondary structures in CRISPR repeats · DOI · quote verified
Cluster differences?
Some clusters fold well; others poorly.
Source: Evolutionary conservation of sequence and secondary structures in CRISPR repeats · DOI · quote verified
Spacer count scale?
Thousands of spacers in the set.
Source: Evolutionary conservation of sequence and secondary structures in CRISPR repeats · DOI · quote verified
CAS context?
CAS gene families accompany CRISPR arrays.
Source: Evolutionary conservation of sequence and secondary structures in CRISPR repeats · DOI · quote verified
Method to find arrays?
PILER-CR on genome set.
Source: Evolutionary conservation of sequence and secondary structures in CRISPR repeats · DOI · quote verified
Takeaway?
CRISPR repeats are sequence/structure-constrained.
Source: Evolutionary conservation of sequence and secondary structures in CRISPR repeats · DOI · quote verified