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Cataloging mammalian circular RNAs
Thousands of mammalian circRNAs arise by back-splicing and can be quantified across ENCODE cell types.
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RNA class?
Mammalian circular RNAs.
Source: Expanded identification and characterization of mammalian circular RNAs · DOI · quote verified
Biogenesis route?
Back-splicing of pre-mRNA.
Source: Expanded identification and characterization of mammalian circular RNAs · DOI · quote verified
Famous sponge example?
CDR1as/ciRS-7 for miR-7.
Source: Expanded identification and characterization of mammalian circular RNAs · DOI · quote verified
Data source?
ENCODE non-poly(A) RNA-seq.
Source: Expanded identification and characterization of mammalian circular RNAs · DOI · quote verified
Detection idea?
Reads spanning reversed exon junctions.
Source: Expanded identification and characterization of mammalian circular RNAs · DOI · quote verified
Canonical splice sites?
Most candidates flanked by GT-AG.
Source: Expanded identification and characterization of mammalian circular RNAs · DOI · quote verified
Noncanonical candidates?
Often low circular fraction.
Source: Expanded identification and characterization of mammalian circular RNAs · DOI · quote verified
Quantification metric?
Circular fraction per locus.
Source: Expanded identification and characterization of mammalian circular RNAs · DOI · quote verified
Why stable sponges?
No free ends for typical decay.
Source: Expanded identification and characterization of mammalian circular RNAs · DOI · quote verified
Takeaway?
circRNAs are a widespread alternative isoform class.
Source: Expanded identification and characterization of mammalian circular RNAs · DOI · quote verified