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Tiling arrays reveal plant circadian RNAs
Genome tiling arrays uncover extensive circadian-regulated transcription across the Arabidopsis genome.
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Organism?
Arabidopsis thaliana.
Source: Exploring the transcriptional landscape of plant circadian rhythms using genome tiling arrays · DOI · quote verified
Platform?
Whole-genome tiling arrays.
Source: Exploring the transcriptional landscape of plant circadian rhythms using genome tiling arrays · DOI · quote verified
Sampling design?
2-day time course at 4-hour resolution.
Source: Exploring the transcriptional landscape of plant circadian rhythms using genome tiling arrays · DOI · quote verified
Analysis?
Spectral tests for circadian hybridization rhythms.
Source: Exploring the transcriptional landscape of plant circadian rhythms using genome tiling arrays · DOI · quote verified
Known clock genes?
PRRs, LUX, ELF3/4, FKF1, ZTL among others.
Source: Exploring the transcriptional landscape of plant circadian rhythms using genome tiling arrays · DOI · quote verified
Beyond coding genes?
Many additional genomic features cycle.
Source: Exploring the transcriptional landscape of plant circadian rhythms using genome tiling arrays · DOI · quote verified
Probe density idea?
Millions of oligonucleotide features.
Source: Exploring the transcriptional landscape of plant circadian rhythms using genome tiling arrays · DOI · quote verified
Protein-coding coverage?
Most annotated genes have exon probes.
Source: Exploring the transcriptional landscape of plant circadian rhythms using genome tiling arrays · DOI · quote verified
Weakly rhythmic clocks?
LKP2 and ZTL behave as expected.
Source: Exploring the transcriptional landscape of plant circadian rhythms using genome tiling arrays · DOI · quote verified
Takeaway?
Plant circadian control is genome-wide, not gene-list limited.
Source: Exploring the transcriptional landscape of plant circadian rhythms using genome tiling arrays · DOI · quote verified