Flashcard library · Biology · PaperFren
Spike glycosylation and palmitoylation trafficking
Glycosylation motifs and cysteine palmitoylation steer SARS-CoV-2 spike maturation, Golgi trafficking, and fusion.
Keep what you just learned
Save these 10 cards to your NoteFren library. Your first review starts right away, and each card links back to the study it came from.
What glycoprotein is studied?
SARS-CoV-2 spike (S).
Source: Glycosylation and S-palmitoylation regulate SARS-CoV-2 spike protein intracellular trafficking · DOI · quote verified
What LYQD mutations were made?
Alanine substitutions (LYAA, AAQD, AAAA).
Source: Glycosylation and S-palmitoylation regulate SARS-CoV-2 spike protein intracellular trafficking · DOI · quote verified
Glycosylation readout for AAQD/AAAA?
Altered S2 electrophoretic mobility versus WT/LYAA.
Source: Glycosylation and S-palmitoylation regulate SARS-CoV-2 spike protein intracellular trafficking · DOI · quote verified
Cellular S forms detected?
Full-length S (~180 kDa) and S2 (~100 kDa).
Source: Glycosylation and S-palmitoylation regulate SARS-CoV-2 spike protein intracellular trafficking · DOI · quote verified
Palmitoylation function class?
Often controls subcellular trafficking.
Source: Glycosylation and S-palmitoylation regulate SARS-CoV-2 spike protein intracellular trafficking · DOI · quote verified
Inhibitor used?
2-bromopalmitate (2BP).
Source: Glycosylation and S-palmitoylation regulate SARS-CoV-2 spike protein intracellular trafficking · DOI · quote verified
2BP effect on production?
Suppresses S pp and SARS-CoV-2 production.
Source: Glycosylation and S-palmitoylation regulate SARS-CoV-2 spike protein intracellular trafficking · DOI · quote verified
Which cysteines matter most?
Clusters I and II for palmitoylation and S pp.
Source: Glycosylation and S-palmitoylation regulate SARS-CoV-2 spike protein intracellular trafficking · DOI · quote verified
Where does palmitoylation send mature S?
Toward Golgi and plasma membrane.
Source: Glycosylation and S-palmitoylation regulate SARS-CoV-2 spike protein intracellular trafficking · DOI · quote verified
Fusion requirement?
Palmitoylation needed for ACE2-mediated cell fusion.
Source: Glycosylation and S-palmitoylation regulate SARS-CoV-2 spike protein intracellular trafficking · DOI · quote verified