Screening reactions in one-second DESI-MS droplets

Desorption electrospray ionization mass spectrometry reads amine alkylation and Suzuki hits from nanoliter spots in about a second.

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How fast does this DESI-MS method read a reaction droplet?

About 1 second per droplet.

How did the two alkyl bromides compare?

Benzyl bromide worked for 7/8 amines; 2-(bromoethyl)benzene for only 3/8.

What peak screening rate was reached?

6004 reactions per hour at 6144-well density.

How small were the spotted mixtures?

50 nL with less than 0.5 μg of each reagent on PTFE.

Which C–C reaction was screened besides alkylation?

Suzuki cross-coupling of phenylboronic acids with aryl halides.

Which palladium precatalyst was used?

Commercial XPhos Pd G3.

What substrate held the spots?

Porous PTFE, spotted with a magnetic pin tool.

How were products confirmed?

Tandem mass spectrometry (MS/MS).

What chemical economy does the method claim?

Minute amounts of chemicals at the low-μg level.

What happens to spots above 10 000 mixtures per hour?

They blur, limiting DESI quality.

How dense can fluorescence-confirmed spots be?

As high as 24 457 per standard plate.