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Hydrophilic freshwater DOM biodegrades fastest

Across a Swedish stream and two lakes, the most water-loving DOM fractions lost 12–25% of DOC in 150 days versus 6–15% for hydrophobic fractions.

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What was the hypothesis?

The most hydrophilic DOM fractions would be the most biodegradable.

Source: Hydrophilic Species Are the Most Biodegradable Components of Freshwater Dissolved Organic Matter · DOI · quote verified

Which waters were sampled?

A humic stream, a clearwater lake, and a eutrophic lake in Uppland, Sweden.

Source: Hydrophilic Species Are the Most Biodegradable Components of Freshwater Dissolved Organic Matter · DOI · quote verified

How was hydrophobicity separated?

C18-bonded silica chromatography retaining hydrophobic species more.

Source: Hydrophilic Species Are the Most Biodegradable Components of Freshwater Dissolved Organic Matter · DOI · quote verified

Which fractions were most labile?

The most hydrophilic fractions (U and 1).

Source: Hydrophilic Species Are the Most Biodegradable Components of Freshwater Dissolved Organic Matter · DOI · quote verified

DOC loss for hydrophilic fractions at day 150?

12–25% DOC loss (k ≥ 0.005).

Source: Hydrophilic Species Are the Most Biodegradable Components of Freshwater Dissolved Organic Matter · DOI · quote verified

DOC loss for hydrophobic fractions?

6–15% DOC loss (k < 0.005); most refractory.

Source: Hydrophilic Species Are the Most Biodegradable Components of Freshwater Dissolved Organic Matter · DOI · quote verified

Did the pattern depend on site type?

No: the hydrophilic-most-labile result was common for all three sites.

Source: Hydrophilic Species Are the Most Biodegradable Components of Freshwater Dissolved Organic Matter · DOI · quote verified

What is the dilution hypothesis?

Compounds persist because each slightly different substrate is vanishingly dilute.

Source: Hydrophilic Species Are the Most Biodegradable Components of Freshwater Dissolved Organic Matter · DOI · quote verified

Why can MS miss hydrophilic DOM?

Extraction and electrospray ionization exclude the most hydrophilic fraction.

Source: Hydrophilic Species Are the Most Biodegradable Components of Freshwater Dissolved Organic Matter · DOI · quote verified

Incubation length?

150 days with repeated DOC sampling.

Source: Hydrophilic Species Are the Most Biodegradable Components of Freshwater Dissolved Organic Matter · DOI · quote verified

Did pooled fractions show strong priming?

Not a consistent interactive effect across all three sites.

Source: Hydrophilic Species Are the Most Biodegradable Components of Freshwater Dissolved Organic Matter · DOI · quote verified