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Native nano-DESI images intact kidney proteins
Ammonium-acetate nano-DESI mass spectrometry imaging maps folded proteins and a noncovalent S100-A6 dimer in rat kidney at finer spatial scale than LESA.
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What ionization method enabled native protein MSI here?
Nano-DESI from nondenaturing ammonium acetate.
Source: Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI · DOI · quote verified
Why keep proteins folded for this experiment?
Charge-state distributions and noncovalent complexes report on native structure.
Source: Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI · DOI · quote verified
What spatial problem did LESA native MSI have?
Coarse sampling: ~600 μm spots and 1 mm pixels.
Source: Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI · DOI · quote verified
Which noncovalent assembly was identified in kidney?
The S100-A6 homodimer.
Source: Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI · DOI · quote verified
What was the largest native protein detected?
The RidA trimer (42.6 kDa).
Source: Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI · DOI · quote verified
What detergent additive was used on kidney?
0.125% C8E4 in 200 mM ammonium acetate.
Source: Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI · DOI · quote verified
How long did kidney ion images take?
About 7 hours.
Source: Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI · DOI · quote verified
What m/z window was used for kidney?
m/z 2000–5000, to skip intense lipid-like singly charged ions.
Source: Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI · DOI · quote verified
What is PTCR used for here?
Deconvoluting overlapping low-resolution protein signals and estimating MW.
Source: Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI · DOI · quote verified
What tissue thickness was analyzed?
10 μm cryosections of rat kidney.
Source: Native Mass Spectrometry Imaging of Proteins and Protein Complexes by Nano-DESI · DOI · quote verified