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Microwave phage carbons glow and sense Fe3+
M13 bacteriophage carbonized in a 700 W microwave gives fluorescent nanoparticles (QY 14.8%) that quench with Fe3+ down to an 8.0 μM LOD.
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What carbon source was used for the nanoparticles?
M13 bacteriophage particles (proteins + DNA).
Source: One-step synthesis of M13 phage-based nanoparticles and their fluorescence properties · DOI · quote verified
How were the particles made in one step?
Microwave heating at 700 W until dry (~5 min).
Source: One-step synthesis of M13 phage-based nanoparticles and their fluorescence properties · DOI · quote verified
What pretreatment denatured the phage?
Stirring with 6 M GuHCl for 3 hours.
Source: One-step synthesis of M13 phage-based nanoparticles and their fluorescence properties · DOI · quote verified
What fluorescence peak pair was used?
Excite at 320 nm, emit at 380 nm.
Source: One-step synthesis of M13 phage-based nanoparticles and their fluorescence properties · DOI · quote verified
What quantum yield was measured?
14.8% versus quinine sulfate.
Source: One-step synthesis of M13 phage-based nanoparticles and their fluorescence properties · DOI · quote verified
What AFM size range was reported?
Diameters from 40 nm to 70 nm.
Source: One-step synthesis of M13 phage-based nanoparticles and their fluorescence properties · DOI · quote verified
Which metal ion was detected by quenching?
Fe3+.
Source: One-step synthesis of M13 phage-based nanoparticles and their fluorescence properties · DOI · quote verified
What Fe3+ LOD was calculated?
8.0 μM.
Source: One-step synthesis of M13 phage-based nanoparticles and their fluorescence properties · DOI · quote verified
Over what Fe3+ range was F0/F linear?
10 μM to 100 μM.
Source: One-step synthesis of M13 phage-based nanoparticles and their fluorescence properties · DOI · quote verified
Why might QY be relatively high?
Nitrogen doping from phage biomolecules.
Source: One-step synthesis of M13 phage-based nanoparticles and their fluorescence properties · DOI · quote verified