Mapping the faces of mitochondria and ER

APEX2 proximity biotinylation yields high-quality proteomic maps of cytosol-facing outer mitochondrial and ER membranes in living human cells.

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What method produced the membrane maps?

APEX2 proximity biotinylation in living cells.

Which mitochondrial membrane face was mapped?

The cytosol-facing outer mitochondrial membrane (OMM).

Which ER membrane processes are highlighted?

Calcium signaling, lipid synthesis, translation, secretion, folding, export/degradation.

How many additional enrichments are noted for OMM vs ERM?

22 in OMM and 72 in ERM proteomes.

Why are the lists useful?

Resources to understand OMM/ERM and generate hypotheses.

What traditional approach is contrasted?

Biochemical fractionation of organelles/subcompartments.

What living-system advantage is implied?

Mapping in living human cells rather than only disrupted fractions.

OMM functions listed include?

Fusion/fission, import, cytoskeleton interactions, apoptosis, innate signaling, organelle contacts.

What was incomplete before?

High-quality proteomic maps of these cytosol-facing membranes.

Student takeaway?

Proximity proteomics can inventory trafficking/signaling membranes in situ.