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How do proteins find plasmodesmata?
PDLP1 is a type I membrane protein targeted to plasmodesmata; altering PDLP dosage changes GFP cell-to-cell trafficking.
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What are plasmodesmata?
Channels crossing the cell wall that establish symplastic continuity.
Source: Specific targeting of a plasmodesmal protein affecting cell-to-cell communication · DOI · quote verified
Why do plasmodesmata matter?
They enable intercellular macromolecular communication and non-cell-autonomous functions.
Source: Specific targeting of a plasmodesmal protein affecting cell-to-cell communication · DOI · quote verified
What protein family targets plasmodesmata here?
Plasmodesmata-Located Protein 1 (PDLP1).
Source: Specific targeting of a plasmodesmal protein affecting cell-to-cell communication · DOI · quote verified
What is PDLP1a’s membrane topology?
Type I: short C-terminal tail in cytoplasm; 2xDUF26 in the apoplast.
Source: Specific targeting of a plasmodesmal protein affecting cell-to-cell communication · DOI · quote verified
What trafficking phenotype do combined PDLP KOs show?
Increased GFP cell-to-cell movement.
Source: Specific targeting of a plasmodesmal protein affecting cell-to-cell communication · DOI · quote verified
Why has identifying plasmodesmal proteins been slow?
Embedded in rigid walls, hindering conventional purification.
Source: Specific targeting of a plasmodesmal protein affecting cell-to-cell communication · DOI · quote verified
Source of candidate proteins?
Highly purified Arabidopsis cell-wall membrane proteome.
Source: Specific targeting of a plasmodesmal protein affecting cell-to-cell communication · DOI · quote verified
Do PDLPs target plasmodesmata in multiple species?
Yes—targeting observed in diverse plant species assays.
Source: Specific targeting of a plasmodesmal protein affecting cell-to-cell communication · DOI · quote verified
What reporter measured communication changes?
GFP movement between cells.
Source: Specific targeting of a plasmodesmal protein affecting cell-to-cell communication · DOI · quote verified
Teaching point?
Specific membrane proteins can be targeted to plasmodesmata and tune cell-to-cell trafficking.
Source: Specific targeting of a plasmodesmal protein affecting cell-to-cell communication · DOI · quote verified
Where is the DUF26 domain relative to the cell?
In the apoplast (outside the plasma membrane).
Source: Specific targeting of a plasmodesmal protein affecting cell-to-cell communication · DOI · quote verified