How specific are bacterial two-component pathways?

Caulobacter histidine kinases prefer their cognate response regulators in vitro; phosphotransfer profiling reveals conserved pathways controlling growth.

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Do Caulobacter histidine kinases prefer cognate regulators?

Yes—strong in vitro kinetic preference for in vivo cognates.

Source: Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis · DOI · quote verified

What method rapidly maps kinase targets?

Phosphotransfer profiling.

Source: Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis · DOI · quote verified

What did profiling show for CC0530?

It preferentially phosphorylates orphan regulator CC3743.

Source: Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis · DOI · quote verified

What is notable about CenK–CenR?

Widely conserved across alphaproteobacteria and linked to growth/envelope integrity.

Source: Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis · DOI · quote verified

What unknown pathway did their toolkit reveal?

A highly conserved two-component pathway essential for C. crescentus growth via cell envelope roles.

Source: Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis · DOI · quote verified

Why does in vitro promiscuity appear sometimes?

Authors suggest long incubations/high concentrations can create artifactual promiscuity.

Source: Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis · DOI · quote verified

Model bacterium?

Caulobacter crescentus.

Source: Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis · DOI · quote verified

Core modules of TCS signaling?

Histidine kinases and response regulators.

Source: Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis · DOI · quote verified

What cellular processes are in the paper’s title focus?

Growth and cell cycle progression.

Source: Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis · DOI · quote verified

Teaching point on specificity?

Cognate kinase–regulator pairs are kinetically preferred, enabling pathway insulation.

Source: Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis · DOI · quote verified

Is CenR conservation strong?

Authors report >60% identity for CenR across alphaproteobacteria alignments.

Source: Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis · DOI · quote verified